Synthesis and evaluation of activity of some novel enaminones derived from poly [4-Hydroxy quinoline 2 [1H]-one Co acrylic acid] against murine schistosomiasis mansoni

In this work, hydrogels based on different ratios of acrylic acid [AAc] and 4-hydroxy quinaline 2 [IH]-one [BDHQ] monomers; were prepared by copolymerization using gamma-rays of [60]Co gamma cell as source. The hydrogels were characterized by versatile techniques. The effect of time absorption, pHs level, temperature, and ionic strength on the absorbency [%] of P [AAc/BDHQ] hydrogels were studied. In the application the prepared hydrogel samples to murine schistosomiasis mansoni field. The results revealed that the percentage of worm's reduction was 56.82% in case of PAAc or comp. I and 69.38% in case of P [AAc/BDHQ] or comp. II. The dead ova were recorded in comp. I and in comp. II were 45.5 +/- 1.11 and 50.25 +/- 1.17, respectively comparing to the control group. Also, It was observed that the Oogram patterns, curative dose of PZQ had Lethal effect on the mature eggs with 100% reduction

Ultrastructural comparison between the effect of secretory / exceretory products of lung stage and the mechanical S. mansoni schistosomula on each of S. mansoni worm and lymphocyte activation

Ultrastructural observations were made to evaluate the effect of secretory/excretory product [SEP] of 7-days old lung stage S. mansoni schistosomula and the mechanical schistosomula on each of S. mansoni worm, lymphocyte activation and humoral immune response in an attempt to develop a protective vaccine with potent efficiency. Multiple doses [100 micro g followed by two booster doses 50 micro g each] at three weeks intervals were injected intraperitoneally into CFW1 SPE albino mice one week prior to expossure to 100 S. mansoni cercariae, mice were sacrified 6 weeks post infection. Separated worms and lymphocytes in peripheral blood, bone marrow and spleen were prepared for electron microscopic [EM] examination. Serum specific anti-schistosomula SEP IgG was measured. EM examination revealed that immunization with lung stage S. mansoni schistosomula [Group I] induced more damage to the S. mansoni worm represented by extend of the degeneration to subtegumental longitudinal muscle layer, some degeneration in the spermatocyts and vacuolation within the testis of male reproductive organ while degeneration was only whithin the tegument in worm treated with mechanical schistosomula [Group II]. Peripheral blood lymphocytes were activated in both groups while bone marrow lymphocytes were activated only in mice treated with SEP of lung stage S. mansoni schistosomula in which more activation of splenic lymphocytes could be detected in the form of increase number of plasma cells. These results confirmed by the highly significant elevation [p<0.01] in the serum level of anti-SEP IgG in group II. In conclusion, our findings showed that multiple intra-peritoneal administration of low doses of SEP released from 7 days-lung schistosomula induced more damage of the S. mansoni worms, activation of lymphocytes and a significant increase serum level of anti-schistosomula SEP IgG than that of mechanical schistosomula